Aromagenesis Workshop 1
Updated: Jun 1, 2022
Laboratory techniques in yeast genetics
VUB Virje Universiteit Brussel
September 3rd - 7th 2018
Marie Sklodovska Curie Initial Training Network
Generation of new yeast strains for improved flavours and aromas in beer and wine
Seminars
Seminar 1 - PCR-based gene targeting HR/NHEJ
Seminar 2 - Introduction to CRISPR-Cas9 technology in yeasts
Seminar 3 - Flocculation in yeasts and industrial beer-making
Practical Classes
PCR-based gene deletion in yeast
CRISPR-Cas9 technology in yeast
Yeast flocculation
Additional molecular biology techniques: genomic DNA preparation from yeast culture, colony PCR, E.coli transformation
Schedule
Monday 3
12 - 14 Welcome and lunch at VUB
14 - 15 Seminar: "PCR-based gene targeting HR/NHRJ"
15 - 18:30 Practical class: Preparation PCR templates and plasmid DNA extraction (Midiprep)
18:30 - 19:30 Free time
19:30 - 21 Visit Brussels: Drink at the Groote Markt
Tuesday 4
8.30 – 9.30 Practical class: Yeast transformation pt.1
9.30 – 10.30 Seminar: “Introduction to CRISPRCas9 technology in yeasts”
10.30 - 12 Practical class: Gel electrophoresis and DNA precipitation with Isopropanol
12 – 13 Lunch break
13 – 15.30 Practical class: Yeast transformation pt.2
15.30 – 18 Visit Brussels: Atomium
18 – 18.30 Practical class: Yeast transformation pt.3
Wednesday 5
9 – 12 Practical class: Genomic DNA preparation
12 – 13 Lunch break
13 – 17 Practical class: PCR from genomic DNA and colony PCR E. coli transformation
17 - 19 Visit Brussels: Team building experience
19 - 21 Dinner in the city center
Thursday 6
9 – 9.30 Practical class: Gel electrophoresis (PCR products Wednesday)
9.30 – 10.30 Seminar: “Flocculation in yeasts”
10.30 – 12 Practical class: Observation of flocculation in S. fermentans
12 – 13 Lunch break
13 - 17 Visit Brussels: Visit tour of Cantillon brewery
Friday 7
9 – 11 Results and discussion of practical classes
11-12 Closing
Virje Universiteit Brussel
Campus Etterbeek
Boulevard de la Plaine, 2
Ixelles 1050
Brussel, Belgium
Contact:
Prof. Dr. Jurgen Wendland
+32-2-6291937
Beatrice Bernardi
+32-2-6291343
Madina Akan
+32-2-6291343