Aromagenesis Workshop 1

Laboratory techniques in yeast genetics


VUB Virje Universiteit Brussel

September 3rd - 7th 2018


Marie Sklodovska Curie Initial Training Network

Generation of new yeast strains for improved flavours and aromas in beer and wine



Seminars

Seminar 1 - PCR-based gene targeting HR/NHEJ
Seminar 2 - Introduction to CRISPR-Cas9 technology in yeasts
Seminar 3 - Flocculation in yeasts and industrial beer-making

Practical Classes

  • PCR-based gene deletion in yeast

  • CRISPR-Cas9 technology in yeast

  • Yeast flocculation

  • Additional molecular biology techniques: genomic DNA preparation from yeast culture, colony PCR, E.coli transformation

Schedule

Monday 3

  • 12 - 14 Welcome and lunch at VUB

  • 14 - 15 Seminar: "PCR-based gene targeting HR/NHRJ"

  • 15 - 18:30 Practical class: Preparation PCR templates and plasmid DNA extraction (Midiprep)

  • 18:30 - 19:30 Free time

  • 19:30 - 21 Visit Brussels: Drink at the Groote Markt

Tuesday 4

  • 8.30 – 9.30 Practical class: Yeast transformation pt.1

  • 9.30 – 10.30 Seminar: “Introduction to CRISPRCas9 technology in yeasts”

  • 10.30 - 12 Practical class: Gel electrophoresis and DNA precipitation with Isopropanol

  • 12 – 13 Lunch break

  • 13 – 15.30 Practical class: Yeast transformation pt.2

  • 15.30 – 18 Visit Brussels: Atomium

  • 18 – 18.30 Practical class: Yeast transformation pt.3

Wednesday 5

  • 9 – 12 Practical class: Genomic DNA preparation

  • 12 – 13 Lunch break

  • 13 – 17 Practical class: PCR from genomic DNA and colony PCR E. coli transformation

  • 17 - 19 Visit Brussels: Team building experience

  • 19 - 21 Dinner in the city center

Thursday 6

  • 9 – 9.30 Practical class: Gel electrophoresis (PCR products Wednesday)

  • 9.30 – 10.30 Seminar: “Flocculation in yeasts”

  • 10.30 – 12 Practical class: Observation of flocculation in S. fermentans

  • 12 – 13 Lunch break

  • 13 - 17 Visit Brussels: Visit tour of Cantillon brewery

Friday 7

  • 9 – 11 Results and discussion of practical classes

  • 11-12 Closing

Virje Universiteit Brussel

Campus Etterbeek

Boulevard de la Plaine, 2

Ixelles 1050

Brussel, Belgium

Contact:

Prof. Dr. Jurgen Wendland

+32-2-6291937

jurgen.wendland@vub.be


Beatrice Bernardi

+32-2-6291343

beatrice.bernardi@vub.be


Madina Akan

+32-2-6291343

madina.akan@vub.be


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